In-Vitro Antibacterial Bioassay of Methanol Extract Fraction (Rf > 0.5) from Rhizophora mucronata L. (Rhizophoraceae) Leaves Against Multidrug-Resistant Escherichia coli
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Abstract
A bioactivity study was conducted in January 2018 on the methanolic extract (Rf > 0.5) of mangrove leaves from Rhizophora mucronata L. (Rhizophoraceae) targeting resistant Escherichia coli. Secondary metabolites were isolated at the Marine Chemical Laboratory, Faculty of Marine and Fisheries, while antibacterial testing was performed at the Microbiology Laboratory, Faculty of Medicine, Syiah Kuala University. Using thin layer chromatography, bioactive fractions were identified, and phytochemical screening confirmed the presence of alkaloids. The extract demonstrated inhibition zones between 7.50–8.50 mm, indicating moderate antibacterial activity against resistant E. coli.
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