Antioxidant and Antibacterial Activity of Ethanol Extract of Senggani Roots (Melastoma malabathricum L.)
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Abstract
This study investigated the antioxidant and antibacterial activities of the ethanol extract of senggani root (Melastoma malabathricumL.), aiming to assess its free radical scavenging potential and its ability to inhibit Escherichia coli. The research involved several stages, including sample preparation, maceration using 96% ethanol, antioxidant testing using the DPPH method at 517 nm, and antibacterial evaluation using the agar diffusion method. Five treatment groups were used in the antibacterial assay: a negative control (sterile distilled water), a positive control (100 µg nystatin), and three extract concentrations (75, 100, and 125 ppm), each tested in triplicate. The results showed that the extract had an IC₅₀ value of 4.67 ppm, which was comparable to that of vitamin C (4.01 ppm), indicating strong antioxidant activity. In antibacterial testing, the extract produced inhibition zones of 11.16 mm, 11.66 mm, and 12.16 mm at the respective concentrations, while the positive control showed a 9.5 mm inhibition zone. These findings suggest that the ethanol extract of senggani root possesses potent antioxidant properties and demonstrates promising antibacterial activity against Escherichia coli.
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