Bioactive Alkaloids from Callyspongia sp.: A Marine Source of Antibacterial Agents Against Drug-Resistant Echerichia coli
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Abstract
This study investigated the antibacterial potential of secondary metabolites from the Indonesian marine sponge Callyspongia sp. against antibiotic-resistant Escherichia coli. Conducted between November 2017 and January 2018, the research involved extraction, isolation, and characterization of bioactive compounds at Syiah Kuala University’s Marine Chemistry, Microbiology, and Instrumentation Laboratories. Isolation was guided by bioactivity, using chromatographic techniques. FTIR spectral analysis identified functional groups indicative of alkaloids, with imine (C=N) absorption at 1637.4 cm⁻¹ and fingerprint confirmation at 1407.2 cm⁻¹. Bioactivity testing showed inhibition zones of 7 mm and 8.25 mm at concentrations of 20 µg/mL and 100 µg/mL, respectively. The positive control, chloramphenicol (35 µg/mL), exhibited a 7.5 mm inhibition zone. These findings suggest that alkaloid compounds from Callyspongia sp. possess promising antibacterial activity comparable to conventional antibiotics. This highlights the potential of Callyspongia sp. as a source of novel antibacterial agents against resistant bacterial strains.
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